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Multi-photon confocal microscope:

Nanoscope has multi-photon confocal microscope built on an Olympus BX61WI Motorized Upright microscope Platform. This system is used for: (i) Confocal imaging of In-Vivo samples, (ii) Intravital Microscopy, (iii) Two-photon microscopy of thick tissue slices, (iv) Patch Clamp Neurophysiology, (v) In-vivo Optoporation, (vi) Optical/Optogenetic stimulation, (vii) Laser axotomy, (viii) Photobleaching (FRAP), (ix) Multi-photon polymerization, and (x) Material processing.

 

The system is equipped with motorized XY stage, motor drive for the objective so as to not interfere with micropipettes, long working distance dry, oil and water immersion objectives. The microscope is equipped for Brightfield, Nomarski Differential Interference Contrast (DIC) and Wide-field Epifluorescence. For Confocal Laser Scanning, there is multi-line Argon laser and Green He-Ne producing laser lines of 405, 458, 488, 543 and 635nm. A Del Mar Photonics Mavericks 65 Tuneable Femtosecond pulsed NIR Laser is integrated to provide laser manipulation and multiphoton capabilities. The near-infrared laser are ideal for manipulating and examining thick tissue samples.

Automated Fluorescence microscopy:

The Discovery-1 system incorporates unique features that enable it to surpass the performance of a conventional automated microscope system. Primary among these unique features are the light path components, which are completely motorized and controlled by software. The filters and light path are designed for maximum light throughput and conservation of image registration between probes. Additionally, the excitation light for the system is more powerful and serviceable than a typical microscope. The liquid light guide acts as a diffuser of the light output from the lamp and delivers an even and intense disc of excitation light to the sample. In addition, the neutral density and excitation wheels are mounted in the light path. The neutral density wheel is mounted inside the lamp housing and the excitation wheel is on the outside for easy access when changing excitation filters. The microscope body is completely controlled by the computer. The only manual control is the focus knob located on the side of the microscope. This knob is electronic and functions only when the “live” mode of the software is activated. The knob is functional only in “live” mode to avoid user input while auto focus routines are running. Once focused on a sample, the microscope remains in the correct focal plane for any objective you select. The system is used for high throughput screening of molecules/cells.

 

MetaMorph® Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior for over 25 years. It is the ideal "glue" for easily integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform meaningful analysis of acquired images. The software offers many user-friendly application modules for biology-specific analysis such as cell signaling, cell counting, and protein expression.

Patch clamp electrophysiology:

The Multiclamp electrophysiology system from Molecular Devices is integrated on an inverted fluorescence microscope. The MultiClamp 700A has many features unlike any other Axon amplifier, including complete computer control (no knobs or buttons); two independent headstages - each capable of either current OR voltage clamp modes; digital mode-switching; multiple feedback resistors in either current or voltage-clamp mode, and automated capacitance and series-resistance compensation.

 

The patch clamp electrophysiology system is integrated with computer-controlled optical stimulation patterns and being used for evaluating efficacy and characteristics of light-sensitive molecules in different cell types.

Upright fluorescence Laser microscope:

The upright fluorescence microscope is coupled with near-infrared laser beam to allow nano-enhanced optical delivery of exogenous impermeable molecules to targeted cells. The system comprises of an EMCCD camera to allow highly-sensitive fluorescence imaging of cells.

Extracellular MEA electrophysiology:

The Multielectrode array electrophysiology system from Plexon allows detection of extracellular electrical activities from 64 channels simultaneously. A computer-controlled multi-wavelength/multi-LED system is integrated with the MEA which allows evaluation of optically-induced electrical activities at varying wavelengths and intensities. The system is being utilized for characterizing light-sensitivity of MCO-sensitized cells.

Tomographic imaging:

The tomographic imaging system utilizes multi-wavelength visible laser interferometer. Tomographic imaging of living/non-living materials is carried out using specialized sample manipulation chamber.

Micro-spectroscopy system:

The upright microscope is integrated with a broadband light source for illumination and fiber-coupled spectrometer for spatial-spectral analysis of samples with high resolution. The system is also capable of simultaneous viewing through eyepiece and imaging.

Slit lamp system for Ophthalmology:

The slit lamp system is being modified to allow better examination of eye using digital imaging technology.

Cell culture Lab:

The cell culture lab is equipped with storage (refrigerators, liquid N2) and culturing of different cell lines.

Biochemical Lab:

The biochemical lab is equipped with general laboratories tools for centrifugation, staining and variety of assays.

Animal housing:

The animal housing allows studies on rodents in a restricted and well-maintained environment under supervision of a veterinarian.

Animal surgery and tissue preparation Lab:

The lab is equipped with autoclave, surgical tools, tissue processing and storage equipment.

General lab:

The general lab area has mechanical, optical, electronic tools and components for prototyping and device development.

6500 Greenville Ave., Ste 740, Dallas, TX 75206  |  Phone: 626-244-5300  |  Fax: 817-719-2692  | Mail: info@nanoscopetech.com

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